Simultaneous determination of tissue tocopherols, tocotrienols, ubiquinols, and ubiquinones

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Simultaneous determination of tissue tocopherols, tocotrienols, ubiquinols, and ubiquinones.

A tissue-specific distribution of the various vitamin E forms, tocotrienols and tocopherols, has been found, suggesting that these forms have unique roles in cellular functions. A sensitive procedure is described for the simultaneous determination of individual tocopherols, tocotrienols, ubiquinols, and ubiquinones using gradient high pressure liquid chromatography (HPLC) and electrochemical de...

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Simultaneous determination of tocotrienols, tocopherols, retinol, and major carotenoids in human plasma.

BACKGROUND Epidemiologic evidence suggests that the concentrations of antioxidant vitamins in human plasma may play an important role in numerous chronic diseases, such as cancer and cardiovascular disease. However, methods for simultaneous measurement of these antioxidants are scarce. We developed and validated a new HPLC method for simultaneous determination of these vitamers in human plasma ...

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Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.

A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed; the best results were obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elut...

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Tocopherols and tocotrienols as vitamin E

Introduction Vitamins are chemical compounds which are components of coenzymes and non-protein ingredients of enzymes. They are essential for life and normal functioning of the body. Vitamins are supplied with food as they cannot be synthesised by the body [1]. Bad diet, impaired vitamin absorption or the use of substances such as caffeine, nicotine and medicines may lead to hypovitaminosis, th...

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Liquid chromatographic determination of tocopherols and tocotrienols in vegetable oils, formulated preparations, and biscuits.

A precise and selective liquid chromatographic procedure for determining tocopherol and tocotrienol isomers in vegetable oils, formulated preparations, and biscuits was developed and validated. The proposed method quantitates vitamin E in better conditions of recoverability and reproducibility than the standard saponification procedure. Tocopherols and tocotrienols were extracted in hexane from...

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 1996

ISSN: 0022-2275

DOI: 10.1016/s0022-2275(20)37587-8